The Effects of Inhibiting P18INK4C Expression on the
Invasion of Gastric Adenocarcinoma Cell Line
( Department of Biochemistry and Molecular Biology, Research Center for Human Gene Therapy, Shanghai Second Medical University, Shanghai 200025, China )
Abstract Using cDNA microarray with double dots of 4096 human genes, P18INK4C, a member of CKI, was found down-regulated in a gastric adenocarcinoma metastatic cell line (RF-48), compared with the corresponding primary cancer cell line (RF-1), which implied that P18INK4C might be involved in cell invasion and metastatic progression of human gastric adenocarcinoma. Antisense RNA expression plasmid was applied to inhibit P18INK4C expression to study the effect of decreased P18INK4C expression on cell migration, invasion and proliferation ability and cell cycle of RF-1. Results showed that inhibition of P18INK4C expression could obviously enhance cell invasion ability of RF-1, but had little effect on its cell cycle and cell migration and proliferation ability. These results implied that P18INK4C might play a pivotal role in regulating cell invasion, rather than regulating cell cycle and proliferation in the progression of human gastric adenocarcinoma as expected before.
Key words P18INK4C; human gastric adenocarcinoma; invasion and metastasis
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